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airway epithelial growth media  (PromoCell)


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    Structured Review

    PromoCell airway epithelial growth media
    mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal <t>epithelial</t> cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).
    Airway Epithelial Growth Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/airway epithelial growth media/product/PromoCell
    Average 97 stars, based on 334 article reviews
    airway epithelial growth media - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Resistance of endothelial cells to SARS-CoV-2 infection in vitro"

    Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

    Journal: Journal of Virology

    doi: 10.1128/jvi.01205-25

    mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal epithelial cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).
    Figure Legend Snippet: mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal epithelial cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).

    Techniques Used: Expressing

    SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).
    Figure Legend Snippet: SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

    Techniques Used: Virus, Infection, Imaging



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    Image Search Results


    mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal epithelial cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).

    Journal: Journal of Virology

    Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

    doi: 10.1128/jvi.01205-25

    Figure Lengend Snippet: mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal epithelial cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).

    Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

    Techniques: Expressing

    SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

    Journal: Journal of Virology

    Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

    doi: 10.1128/jvi.01205-25

    Figure Lengend Snippet: SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

    Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

    Techniques: Virus, Infection, Imaging